Synthesized:Article Title: Fibroblast-derived Collagen VI shapes the structure and function of the tumor-immune microenvironment in clear cell renal cell carcinoma
Article Snippet: Human renal cancer cell lines A498 (HTB-44, ATCC,) and 786-O (CRL-1932, ATCC) as well as human renal fibroblasts TK173 (RRID: CVCL_C8FA) and human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031) were used and obtained as recently described .. A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).
Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
Article Snippet: Human renal cancer cell lines A498 (white female donor, ATCC, HTB-44) and 786-O (white male donor, ATCC, CRL-1932) (both, WT and with VHL re-expression) as well as human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031, male donor) were used and obtained as recently described.. A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).
Functional Assay:Article Title: Fibroblast-derived Collagen VI shapes the structure and function of the tumor-immune microenvironment in clear cell renal cell carcinoma
Article Snippet: Human renal cancer cell lines A498 (HTB-44, ATCC,) and 786-O (CRL-1932, ATCC) as well as human renal fibroblasts TK173 (RRID: CVCL_C8FA) and human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031) were used and obtained as recently described .. A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).
Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
Article Snippet: Human renal cancer cell lines A498 (white female donor, ATCC, HTB-44) and 786-O (white male donor, ATCC, CRL-1932) (both, WT and with VHL re-expression) as well as human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031, male donor) were used and obtained as recently described.. A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).
RNA Sequencing:Article Title: Fibroblast-derived Collagen VI shapes the structure and function of the tumor-immune microenvironment in clear cell renal cell carcinoma
Article Snippet: Human renal cancer cell lines A498 (HTB-44, ATCC,) and 786-O (CRL-1932, ATCC) as well as human renal fibroblasts TK173 (RRID: CVCL_C8FA) and human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031) were used and obtained as recently described .. A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).
Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
Article Snippet: Human renal cancer cell lines A498 (white female donor, ATCC, HTB-44) and 786-O (white male donor, ATCC, CRL-1932) (both, WT and with VHL re-expression) as well as human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031, male donor) were used and obtained as recently described.. A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).
Staining:Article Title: Fibroblast-derived Collagen VI shapes the structure and function of the tumor-immune microenvironment in clear cell renal cell carcinoma
Article Snippet: Human renal cancer cell lines A498 (HTB-44, ATCC,) and 786-O (CRL-1932, ATCC) as well as human renal fibroblasts TK173 (RRID: CVCL_C8FA) and human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031) were used and obtained as recently described .. A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).
Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
Article Snippet: Human renal cancer cell lines A498 (white female donor, ATCC, HTB-44) and 786-O (white male donor, ATCC, CRL-1932) (both, WT and with VHL re-expression) as well as human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031, male donor) were used and obtained as recently described.. A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).
Electron Microscopy:Article Title: Fibroblast-derived Collagen VI shapes the structure and function of the tumor-immune microenvironment in clear cell renal cell carcinoma
Article Snippet: Human renal cancer cell lines A498 (HTB-44, ATCC,) and 786-O (CRL-1932, ATCC) as well as human renal fibroblasts TK173 (RRID: CVCL_C8FA) and human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031) were used and obtained as recently described .. A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).
Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
Article Snippet: Human renal cancer cell lines A498 (white female donor, ATCC, HTB-44) and 786-O (white male donor, ATCC, CRL-1932) (both, WT and with VHL re-expression) as well as human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031, male donor) were used and obtained as recently described.. A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).
Marker:Article Title: Fibroblast-derived Collagen VI shapes the structure and function of the tumor-immune microenvironment in clear cell renal cell carcinoma
Article Snippet: Human renal cancer cell lines A498 (HTB-44, ATCC,) and 786-O (CRL-1932, ATCC) as well as human renal fibroblasts TK173 (RRID: CVCL_C8FA) and human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031) were used and obtained as recently described .. A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).
Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
Article Snippet: Human renal cancer cell lines A498 (white female donor, ATCC, HTB-44) and 786-O (white male donor, ATCC, CRL-1932) (both, WT and with VHL re-expression) as well as human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031, male donor) were used and obtained as recently described.. A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).
Spatial Transcriptomics:Article Title: Fibroblast-derived Collagen VI shapes the structure and function of the tumor-immune microenvironment in clear cell renal cell carcinoma
Article Snippet: Human renal cancer cell lines A498 (HTB-44, ATCC,) and 786-O (CRL-1932, ATCC) as well as human renal fibroblasts TK173 (RRID: CVCL_C8FA) and human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031) were used and obtained as recently described .. A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).
Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
Article Snippet: Human renal cancer cell lines A498 (white female donor, ATCC, HTB-44) and 786-O (white male donor, ATCC, CRL-1932) (both, WT and with VHL re-expression) as well as human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031, male donor) were used and obtained as recently described.. A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).
Expressing:Article Title: Fibroblast-derived Collagen VI shapes the structure and function of the tumor-immune microenvironment in clear cell renal cell carcinoma
Article Snippet: Human renal cancer cell lines A498 (HTB-44, ATCC,) and 786-O (CRL-1932, ATCC) as well as human renal fibroblasts TK173 (RRID: CVCL_C8FA) and human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031) were used and obtained as recently described .. A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).
Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
Article Snippet: Human renal cancer cell lines A498 (white female donor, ATCC, HTB-44) and 786-O (white male donor, ATCC, CRL-1932) (both, WT and with VHL re-expression) as well as human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031, male donor) were used and obtained as recently described.. A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).
Derivative Assay:Article Title: Fibroblast-derived Collagen VI shapes the structure and function of the tumor-immune microenvironment in clear cell renal cell carcinoma
Article Snippet: Human renal cancer cell lines A498 (HTB-44, ATCC,) and 786-O (CRL-1932, ATCC) as well as human renal fibroblasts TK173 (RRID: CVCL_C8FA) and human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031) were used and obtained as recently described .. A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).
Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
Article Snippet: Human renal cancer cell lines A498 (white female donor, ATCC, HTB-44) and 786-O (white male donor, ATCC, CRL-1932) (both, WT and with VHL re-expression) as well as human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031, male donor) were used and obtained as recently described.. A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).
Western Blot:Article Title: Fibroblast-derived Collagen VI shapes the structure and function of the tumor-immune microenvironment in clear cell renal cell carcinoma
Article Snippet: Human renal cancer cell lines A498 (HTB-44, ATCC,) and 786-O (CRL-1932, ATCC) as well as human renal fibroblasts TK173 (RRID: CVCL_C8FA) and human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031) were used and obtained as recently described .. A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).
Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
Article Snippet: Human renal cancer cell lines A498 (white female donor, ATCC, HTB-44) and 786-O (white male donor, ATCC, CRL-1932) (both, WT and with VHL re-expression) as well as human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031, male donor) were used and obtained as recently described.. A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).
shRNA:Article Title: Fibroblast-derived Collagen VI shapes the structure and function of the tumor-immune microenvironment in clear cell renal cell carcinoma
Article Snippet: Human renal cancer cell lines A498 (HTB-44, ATCC,) and 786-O (CRL-1932, ATCC) as well as human renal fibroblasts TK173 (RRID: CVCL_C8FA) and human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031) were used and obtained as recently described .. A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).
Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
Article Snippet: Human renal cancer cell lines A498 (white female donor, ATCC, HTB-44) and 786-O (white male donor, ATCC, CRL-1932) (both, WT and with VHL re-expression) as well as human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031, male donor) were used and obtained as recently described.. A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).
Knockdown:Article Title: Fibroblast-derived Collagen VI shapes the structure and function of the tumor-immune microenvironment in clear cell renal cell carcinoma
Article Snippet: Human renal cancer cell lines A498 (HTB-44, ATCC,) and 786-O (CRL-1932, ATCC) as well as human renal fibroblasts TK173 (RRID: CVCL_C8FA) and human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031) were used and obtained as recently described .. A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).
Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
Article Snippet: Human renal cancer cell lines A498 (white female donor, ATCC, HTB-44) and 786-O (white male donor, ATCC, CRL-1932) (both, WT and with VHL re-expression) as well as human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031, male donor) were used and obtained as recently described.. A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).
Over Expression:Article Title: Fibroblast-derived Collagen VI shapes the structure and function of the tumor-immune microenvironment in clear cell renal cell carcinoma
Article Snippet: Human renal cancer cell lines A498 (HTB-44, ATCC,) and 786-O (CRL-1932, ATCC) as well as human renal fibroblasts TK173 (RRID: CVCL_C8FA) and human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031) were used and obtained as recently described .. A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).
Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
Article Snippet: Human renal cancer cell lines A498 (white female donor, ATCC, HTB-44) and 786-O (white male donor, ATCC, CRL-1932) (both, WT and with VHL re-expression) as well as human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031, male donor) were used and obtained as recently described.. A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).
Control:Article Title: Fibroblast-derived Collagen VI shapes the structure and function of the tumor-immune microenvironment in clear cell renal cell carcinoma
Article Snippet: Human renal cancer cell lines A498 (HTB-44, ATCC,) and 786-O (CRL-1932, ATCC) as well as human renal fibroblasts TK173 (RRID: CVCL_C8FA) and human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031) were used and obtained as recently described .. A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).
Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
Article Snippet: Human renal cancer cell lines A498 (white female donor, ATCC, HTB-44) and 786-O (white male donor, ATCC, CRL-1932) (both, WT and with VHL re-expression) as well as human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031, male donor) were used and obtained as recently described.. A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).
Luciferase:Article Title: Fibroblast-derived Collagen VI shapes the structure and function of the tumor-immune microenvironment in clear cell renal cell carcinoma
Article Snippet: Human renal cancer cell lines A498 (HTB-44, ATCC,) and 786-O (CRL-1932, ATCC) as well as human renal fibroblasts TK173 (RRID: CVCL_C8FA) and human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031) were used and obtained as recently described .. A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).
Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
Article Snippet: Human renal cancer cell lines A498 (white female donor, ATCC, HTB-44) and 786-O (white male donor, ATCC, CRL-1932) (both, WT and with VHL re-expression) as well as human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031, male donor) were used and obtained as recently described.. A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).
Knock-Out:Article Title: Fibroblast-derived Collagen VI shapes the structure and function of the tumor-immune microenvironment in clear cell renal cell carcinoma
Article Snippet: Human renal cancer cell lines A498 (HTB-44, ATCC,) and 786-O (CRL-1932, ATCC) as well as human renal fibroblasts TK173 (RRID: CVCL_C8FA) and human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031) were used and obtained as recently described .. A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).
Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
Article Snippet: Human renal cancer cell lines A498 (white female donor, ATCC, HTB-44) and 786-O (white male donor, ATCC, CRL-1932) (both, WT and with VHL re-expression) as well as human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031, male donor) were used and obtained as recently described.. A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).
CRISPR:Article Title: Fibroblast-derived Collagen VI shapes the structure and function of the tumor-immune microenvironment in clear cell renal cell carcinoma
Article Snippet: Human renal cancer cell lines A498 (HTB-44, ATCC,) and 786-O (CRL-1932, ATCC) as well as human renal fibroblasts TK173 (RRID: CVCL_C8FA) and human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031) were used and obtained as recently described .. A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).
Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
Article Snippet: Human renal cancer cell lines A498 (white female donor, ATCC, HTB-44) and 786-O (white male donor, ATCC, CRL-1932) (both, WT and with VHL re-expression) as well as human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031, male donor) were used and obtained as recently described.. A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).
Generated:Article Title: Fibroblast-derived Collagen VI shapes the structure and function of the tumor-immune microenvironment in clear cell renal cell carcinoma
Article Snippet: Human renal cancer cell lines A498 (HTB-44, ATCC,) and 786-O (CRL-1932, ATCC) as well as human renal fibroblasts TK173 (RRID: CVCL_C8FA) and human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031) were used and obtained as recently described .. A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).
Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
Article Snippet: Human renal cancer cell lines A498 (white female donor, ATCC, HTB-44) and 786-O (white male donor, ATCC, CRL-1932) (both, WT and with VHL re-expression) as well as human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031, male donor) were used and obtained as recently described.. A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).
BrdU Incorporation Assay:Article Title: Fibroblast-derived Collagen VI shapes the structure and function of the tumor-immune microenvironment in clear cell renal cell carcinoma
Article Snippet: Human renal cancer cell lines A498 (HTB-44, ATCC,) and 786-O (CRL-1932, ATCC) as well as human renal fibroblasts TK173 (RRID: CVCL_C8FA) and human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031) were used and obtained as recently described .. A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).
Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
Article Snippet: Human renal cancer cell lines A498 (white female donor, ATCC, HTB-44) and 786-O (white male donor, ATCC, CRL-1932) (both, WT and with VHL re-expression) as well as human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031, male donor) were used and obtained as recently described.. A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).
Membrane:Article Title: Fibroblast-derived Collagen VI shapes the structure and function of the tumor-immune microenvironment in clear cell renal cell carcinoma
Article Snippet: Human renal cancer cell lines A498 (HTB-44, ATCC,) and 786-O (CRL-1932, ATCC) as well as human renal fibroblasts TK173 (RRID: CVCL_C8FA) and human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031) were used and obtained as recently described .. A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).
Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
Article Snippet: Human renal cancer cell lines A498 (white female donor, ATCC, HTB-44) and 786-O (white male donor, ATCC, CRL-1932) (both, WT and with VHL re-expression) as well as human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031, male donor) were used and obtained as recently described.. A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).
Immunofluorescence:Article Title: Fibroblast-derived Collagen VI shapes the structure and function of the tumor-immune microenvironment in clear cell renal cell carcinoma
Article Snippet: Human renal cancer cell lines A498 (HTB-44, ATCC,) and 786-O (CRL-1932, ATCC) as well as human renal fibroblasts TK173 (RRID: CVCL_C8FA) and human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031) were used and obtained as recently described .. A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).
Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
Article Snippet: Human renal cancer cell lines A498 (white female donor, ATCC, HTB-44) and 786-O (white male donor, ATCC, CRL-1932) (both, WT and with VHL re-expression) as well as human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031, male donor) were used and obtained as recently described.. A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).
Fluorescence:Article Title: Fibroblast-derived Collagen VI shapes the structure and function of the tumor-immune microenvironment in clear cell renal cell carcinoma
Article Snippet: Human renal cancer cell lines A498 (HTB-44, ATCC,) and 786-O (CRL-1932, ATCC) as well as human renal fibroblasts TK173 (RRID: CVCL_C8FA) and human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031) were used and obtained as recently described .. A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).
Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
Article Snippet: Human renal cancer cell lines A498 (white female donor, ATCC, HTB-44) and 786-O (white male donor, ATCC, CRL-1932) (both, WT and with VHL re-expression) as well as human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031, male donor) were used and obtained as recently described.. A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).
Negative Control:Article Title: Fibroblast-derived Collagen VI shapes the structure and function of the tumor-immune microenvironment in clear cell renal cell carcinoma
Article Snippet: Human renal cancer cell lines A498 (HTB-44, ATCC,) and 786-O (CRL-1932, ATCC) as well as human renal fibroblasts TK173 (RRID: CVCL_C8FA) and human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031) were used and obtained as recently described .. A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).
Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
Article Snippet: Human renal cancer cell lines A498 (white female donor, ATCC, HTB-44) and 786-O (white male donor, ATCC, CRL-1932) (both, WT and with VHL re-expression) as well as human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031, male donor) were used and obtained as recently described.. A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).
Clinical Proteomics:Article Title: Fibroblast-derived Collagen VI shapes the structure and function of the tumor-immune microenvironment in clear cell renal cell carcinoma
Article Snippet: Human renal cancer cell lines A498 (HTB-44, ATCC,) and 786-O (CRL-1932, ATCC) as well as human renal fibroblasts TK173 (RRID: CVCL_C8FA) and human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031) were used and obtained as recently described .. A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).
Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
Article Snippet: Human renal cancer cell lines A498 (white female donor, ATCC, HTB-44) and 786-O (white male donor, ATCC, CRL-1932) (both, WT and with VHL re-expression) as well as human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031, male donor) were used and obtained as recently described.. A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).
Immunohistochemistry:Article Title: Fibroblast-derived Collagen VI shapes the structure and function of the tumor-immune microenvironment in clear cell renal cell carcinoma
Article Snippet: Human renal cancer cell lines A498 (HTB-44, ATCC,) and 786-O (CRL-1932, ATCC) as well as human renal fibroblasts TK173 (RRID: CVCL_C8FA) and human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031) were used and obtained as recently described .. A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).
Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
Article Snippet: Human renal cancer cell lines A498 (white female donor, ATCC, HTB-44) and 786-O (white male donor, ATCC, CRL-1932) (both, WT and with VHL re-expression) as well as human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031, male donor) were used and obtained as recently described.. A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).
Sequencing:Article Title: Fibroblast-derived Collagen VI shapes the structure and function of the tumor-immune microenvironment in clear cell renal cell carcinoma
Article Snippet: Human renal cancer cell lines A498 (HTB-44, ATCC,) and 786-O (CRL-1932, ATCC) as well as human renal fibroblasts TK173 (RRID: CVCL_C8FA) and human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031) were used and obtained as recently described .. A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).
Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
Article Snippet: Human renal cancer cell lines A498 (white female donor, ATCC, HTB-44) and 786-O (white male donor, ATCC, CRL-1932) (both, WT and with VHL re-expression) as well as human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031, male donor) were used and obtained as recently described.. A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).
Clone Assay:Article Title: Fibroblast-derived Collagen VI shapes the structure and function of the tumor-immune microenvironment in clear cell renal cell carcinoma
Article Snippet: Human renal cancer cell lines A498 (HTB-44, ATCC,) and 786-O (CRL-1932, ATCC) as well as human renal fibroblasts TK173 (RRID: CVCL_C8FA) and human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031) were used and obtained as recently described .. A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).
Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
Article Snippet: Human renal cancer cell lines A498 (white female donor, ATCC, HTB-44) and 786-O (white male donor, ATCC, CRL-1932) (both, WT and with VHL re-expression) as well as human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031, male donor) were used and obtained as recently described.. A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).
Quantitative Proteomics:Article Title: Fibroblast-derived Collagen VI shapes the structure and function of the tumor-immune microenvironment in clear cell renal cell carcinoma
Article Snippet: Human renal cancer cell lines A498 (HTB-44, ATCC,) and 786-O (CRL-1932, ATCC) as well as human renal fibroblasts TK173 (RRID: CVCL_C8FA) and human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031) were used and obtained as recently described .. A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).
Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
Article Snippet: Human renal cancer cell lines A498 (white female donor, ATCC, HTB-44) and 786-O (white male donor, ATCC, CRL-1932) (both, WT and with VHL re-expression) as well as human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031, male donor) were used and obtained as recently described.. A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).
Migration:Article Title: Fibroblast-derived Collagen VI shapes the structure and function of the tumor-immune microenvironment in clear cell renal cell carcinoma
Article Snippet: Human renal cancer cell lines A498 (HTB-44, ATCC,) and 786-O (CRL-1932, ATCC) as well as human renal fibroblasts TK173 (RRID: CVCL_C8FA) and human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031) were used and obtained as recently described .. A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).
Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
Article Snippet: Human renal cancer cell lines A498 (white female donor, ATCC, HTB-44) and 786-O (white male donor, ATCC, CRL-1932) (both, WT and with VHL re-expression) as well as human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031, male donor) were used and obtained as recently described.. A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).
Virus:Article Title: Fibroblast-derived Collagen VI shapes the structure and function of the tumor-immune microenvironment in clear cell renal cell carcinoma
Article Snippet: Human renal cancer cell lines A498 (HTB-44, ATCC,) and 786-O (CRL-1932, ATCC) as well as human renal fibroblasts TK173 (RRID: CVCL_C8FA) and human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031) were used and obtained as recently described .. A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).
Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
Article Snippet: Human renal cancer cell lines A498 (white female donor, ATCC, HTB-44) and 786-O (white male donor, ATCC, CRL-1932) (both, WT and with VHL re-expression) as well as human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031, male donor) were used and obtained as recently described.. A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).
Recombinant:Article Title: Fibroblast-derived Collagen VI shapes the structure and function of the tumor-immune microenvironment in clear cell renal cell carcinoma
Article Snippet: Human renal cancer cell lines A498 (HTB-44, ATCC,) and 786-O (CRL-1932, ATCC) as well as human renal fibroblasts TK173 (RRID: CVCL_C8FA) and human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031) were used and obtained as recently described .. A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).
Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
Article Snippet: Human renal cancer cell lines A498 (white female donor, ATCC, HTB-44) and 786-O (white male donor, ATCC, CRL-1932) (both, WT and with VHL re-expression) as well as human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031, male donor) were used and obtained as recently described.. A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).
Saline:Article Title: Fibroblast-derived Collagen VI shapes the structure and function of the tumor-immune microenvironment in clear cell renal cell carcinoma
Article Snippet: Human renal cancer cell lines A498 (HTB-44, ATCC,) and 786-O (CRL-1932, ATCC) as well as human renal fibroblasts TK173 (RRID: CVCL_C8FA) and human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031) were used and obtained as recently described .. A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).
Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
Article Snippet: Human renal cancer cell lines A498 (white female donor, ATCC, HTB-44) and 786-O (white male donor, ATCC, CRL-1932) (both, WT and with VHL re-expression) as well as human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031, male donor) were used and obtained as recently described.. A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).
Enzyme-linked Immunosorbent Assay:Article Title: Fibroblast-derived Collagen VI shapes the structure and function of the tumor-immune microenvironment in clear cell renal cell carcinoma
Article Snippet: Human renal cancer cell lines A498 (HTB-44, ATCC,) and 786-O (CRL-1932, ATCC) as well as human renal fibroblasts TK173 (RRID: CVCL_C8FA) and human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031) were used and obtained as recently described .. A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).
Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
Article Snippet: Human renal cancer cell lines A498 (white female donor, ATCC, HTB-44) and 786-O (white male donor, ATCC, CRL-1932) (both, WT and with VHL re-expression) as well as human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031, male donor) were used and obtained as recently described.. A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).
Bicinchoninic Acid Protein Assay:Article Title: Fibroblast-derived Collagen VI shapes the structure and function of the tumor-immune microenvironment in clear cell renal cell carcinoma
Article Snippet: Human renal cancer cell lines A498 (HTB-44, ATCC,) and 786-O (CRL-1932, ATCC) as well as human renal fibroblasts TK173 (RRID: CVCL_C8FA) and human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031) were used and obtained as recently described .. A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).
Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
Article Snippet: Human renal cancer cell lines A498 (white female donor, ATCC, HTB-44) and 786-O (white male donor, ATCC, CRL-1932) (both, WT and with VHL re-expression) as well as human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031, male donor) were used and obtained as recently described.. A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).
Gene Expression:Article Title: Fibroblast-derived Collagen VI shapes the structure and function of the tumor-immune microenvironment in clear cell renal cell carcinoma
Article Snippet: Human renal cancer cell lines A498 (HTB-44, ATCC,) and 786-O (CRL-1932, ATCC) as well as human renal fibroblasts TK173 (RRID: CVCL_C8FA) and human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031) were used and obtained as recently described .. A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).
Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
Article Snippet: Human renal cancer cell lines A498 (white female donor, ATCC, HTB-44) and 786-O (white male donor, ATCC, CRL-1932) (both, WT and with VHL re-expression) as well as human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031, male donor) were used and obtained as recently described.. A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).
Imaging:Article Title: Fibroblast-derived Collagen VI shapes the structure and function of the tumor-immune microenvironment in clear cell renal cell carcinoma
Article Snippet: Human renal cancer cell lines A498 (HTB-44, ATCC,) and 786-O (CRL-1932, ATCC) as well as human renal fibroblasts TK173 (RRID: CVCL_C8FA) and human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031) were used and obtained as recently described .. A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).
Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
Article Snippet: Human renal cancer cell lines A498 (white female donor, ATCC, HTB-44) and 786-O (white male donor, ATCC, CRL-1932) (both, WT and with VHL re-expression) as well as human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031, male donor) were used and obtained as recently described.. A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).
Plasmid Preparation:Article Title: Fibroblast-derived Collagen VI shapes the structure and function of the tumor-immune microenvironment in clear cell renal cell carcinoma
Article Snippet: Human renal cancer cell lines A498 (HTB-44, ATCC,) and 786-O (CRL-1932, ATCC) as well as human renal fibroblasts TK173 (RRID: CVCL_C8FA) and human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031) were used and obtained as recently described .. A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).
Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
Article Snippet: Human renal cancer cell lines A498 (white female donor, ATCC, HTB-44) and 786-O (white male donor, ATCC, CRL-1932) (both, WT and with VHL re-expression) as well as human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031, male donor) were used and obtained as recently described.. A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).
Cloning:Article Title: Fibroblast-derived Collagen VI shapes the structure and function of the tumor-immune microenvironment in clear cell renal cell carcinoma
Article Snippet: Human renal cancer cell lines A498 (HTB-44, ATCC,) and 786-O (CRL-1932, ATCC) as well as human renal fibroblasts TK173 (RRID: CVCL_C8FA) and human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031) were used and obtained as recently described .. A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).
Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
Article Snippet: Human renal cancer cell lines A498 (white female donor, ATCC, HTB-44) and 786-O (white male donor, ATCC, CRL-1932) (both, WT and with VHL re-expression) as well as human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031, male donor) were used and obtained as recently described.. A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).
Software:Article Title: Fibroblast-derived Collagen VI shapes the structure and function of the tumor-immune microenvironment in clear cell renal cell carcinoma
Article Snippet: Human renal cancer cell lines A498 (HTB-44, ATCC,) and 786-O (CRL-1932, ATCC) as well as human renal fibroblasts TK173 (RRID: CVCL_C8FA) and human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031) were used and obtained as recently described .. A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).
Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
Article Snippet: Human renal cancer cell lines A498 (white female donor, ATCC, HTB-44) and 786-O (white male donor, ATCC, CRL-1932) (both, WT and with VHL re-expression) as well as human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031, male donor) were used and obtained as recently described.. A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).
Cell Analysis:Article Title: Fibroblast-derived Collagen VI shapes the structure and function of the tumor-immune microenvironment in clear cell renal cell carcinoma
Article Snippet: Human renal cancer cell lines A498 (HTB-44, ATCC,) and 786-O (CRL-1932, ATCC) as well as human renal fibroblasts TK173 (RRID: CVCL_C8FA) and human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031) were used and obtained as recently described .. A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).
Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
Article Snippet: Human renal cancer cell lines A498 (white female donor, ATCC, HTB-44) and 786-O (white male donor, ATCC, CRL-1932) (both, WT and with VHL re-expression) as well as human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031, male donor) were used and obtained as recently described.. A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).
Microscopy:Article Title: Fibroblast-derived Collagen VI shapes the structure and function of the tumor-immune microenvironment in clear cell renal cell carcinoma
Article Snippet: Human renal cancer cell lines A498 (HTB-44, ATCC,) and 786-O (CRL-1932, ATCC) as well as human renal fibroblasts TK173 (RRID: CVCL_C8FA) and human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031) were used and obtained as recently described .. A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).
Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
Article Snippet: Human renal cancer cell lines A498 (white female donor, ATCC, HTB-44) and 786-O (white male donor, ATCC, CRL-1932) (both, WT and with VHL re-expression) as well as human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031, male donor) were used and obtained as recently described.. A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).
Cell Culture:Article Title: Fibroblast-derived Collagen VI shapes the structure and function of the tumor-immune microenvironment in clear cell renal cell carcinoma
Article Snippet: Human renal cancer cell lines A498 (HTB-44, ATCC,) and 786-O (CRL-1932, ATCC) as well as human renal fibroblasts TK173 (RRID: CVCL_C8FA) and human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031) were used and obtained as recently described .. A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).A498, 786-O, TK173 and RPTEC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 (61870036, Thermo Fisher Scientific) supplemented with 10% FCS (S0615, Sigma-Aldrich) and 1% penicillin-streptomycin (P/S, 15140122, Thermo Fisher Scientific).
Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
Article Snippet: Human renal cancer cell lines A498 (white female donor, ATCC, HTB-44) and 786-O (white male donor, ATCC, CRL-1932) (both, WT and with VHL re-expression) as well as human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031, male donor) were used and obtained as recently described.. A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).A498, 786-O and hRPETC cell lines were cultured below 80% confluence using culture medium comprising RPMI-1640 GlutaMAX (Thermo Fisher Scientific Inc., Waltham, MA, USA, #61870036) supplemented with 10% FCS (Merck KGaA, Darmstadt, Germany, #S0615) and 1% penicillin-streptomycin (Thermo Fisher Scientific, #15140122).
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